During the previous step, begin to prepare Whatman paper and nylon membrane for the transfer.Rinse with water, then wash with neutralization solution.Place the gel in a container with denaturing solution, and wash twice for 15 minutes on a shaker.Load samples into wells and include a DNA molecular weight marker. Gel electrophoresis: prepare an agarose gel and either TAE or TBE buffer (buffer selection will depend on the duration of the run and the size of the DNA fragments).Restriction digestion: digest the DNA with a restriction enzyme, and if necessary, concentrate digested DNA.Detection methods differ based on the probe label radiolabeled probes are visualized with X-ray film or phosphorimaging, and enzymatically labeled probes are visualized with chemiluminescent substrate. The fully hybridized labeled probe molecules will remain bound to the blot. Hybridization of complementary sequences occurs during incubation, and the unhybridized probe is removed by washing with buffer. The membrane is incubated with a nucleic acid probe that has a sequence homologous to the target sequence and is labeled with radioactivity, fluorescent dye, or an enzyme capable of generating a chemiluminescent signal. Following electrophoresis, the DNA on the gel is transferred to a nylon membrane. Smaller fragments will migrate farther on the gel than larger ones. The fragments are separated by size on an agarose or polyacrylamide gel via electrophoresis. The southern blot protocol begins with DNA extraction from the cells or tissues, which is then enzymatically digested to produce DNA fragments. Southern blots are used to determine the identity, size, and abundance of specific DNA sequences. Blotting techniques are selected based on the target molecule: DNA, RNA, or protein. This enables radiolabeled or enzymatically labeled antibody or DNA probes to bind the immobilized target, and the molecules of interest may then be visualized with various methods. Southern, northern, and western blot protocols are similar, and begin with electrophoretic separation of protein and nucleic acid fragments on a gel, which are then transferred to a membrane (nitrocellulose membrane, polyvinylidene difluoride (PVDF) membrane, etc.) where they are immobilized. PDF Version What’s the difference between a Southern, Northern, and Western Blot?ĭifferent blotting techniques are used to identify unique proteins and nucleic acid sequences.
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There is an option that lets you extract the email that contains a specific string value in it only and discard the rest emails from the output. You can extract emails from a large text file, doc, docx, html, pdf very easily and the emails extracted will be displayed on the output window, from where you can copy those email addresses for further marketing and advert useĮmail Extractor Lite 1.8 online tool provides you the facility to filter the data according to the query you specify. The email software will now separate the good emails after extraction and will display all the emails that do not contain the specified string value in it. There is also an interface on Lite1.8 or Lite.8 which allows you to extract the email that contains a specific string value in it only and the rest of the emails would be filtered out from the output. This excellent online tool or application offers you the feature to filter email addresses from any data according to the settings you choose. Another feature is the option to sort the emails, which will display the extracted emails alphabetically and the feature to convert the emails to lower case is also integrated. Next feature is grouping emails, which allow you to view the extracted emails in any number of groups as per your requirements. You can also specify any parameter you prefer for the way the data will be separated. One of the best feature is separator which allows you to separate the emails the way you want your emails to be separated from each other. It allows you to modify the output result in a way you want. Multiple Formatted Output Options in Lite1.8Įmail Extractor Lite1.8 has multiple output option, a features you will only see in premium software and tools. So you can extract emails from a large text very easily and the emails extracted will be displayed on the output window, from where you can copy those email addresses for further use. There is no word limit to the text that needs to be entered in the input window. There are 2 windows, output window and input window on the top of the Lite 1.8 Extractor, one is for input and the other will display the output. The interface of the software tool is very easy and user-friendly to operate for a novice. Working with Lite 1.8 email extractorĮmail Extractor Lite 1.8 make the task much simpler and efficient for any individual or business. If an individual sits down to extract emails manually from the records of his customers, it would take hours of hard work and efforts for such a simple task as he has to go through large texts thoroughly which would drain out his energy as well. It can be convenient for individuals and businesses who require emails from their customers information stored in a database. This Lite 1.8 tool is very powerful and easy to use. it is the same as lite 1.8.1 Email Extractor Lite1.8Įmail Extractor Lite 1.8 is a freeware tool which can extract any number of emails from large texts very easily and quickly. It is a great tool for creating your customer contact list using your mailbox data. It is a lightweight and powerful utility designed to extract email addresses from various sources: local files, websites, search engines, etc. Email Extractor 1.8 is free all-in-one freeware online software. To effectively reenergize their workforces, organizations need to shift their emphasis from getting more out of people to investing more in them, so they are motivated-and able-to bring more of themselves to work every day. In each, energy can be systematically expanded and regularly renewed by establishing specific rituals-behaviors that are intentionally practiced and precisely scheduled, with the goal of making them unconscious and automatic as quickly as possible. Defined in physics as the capacity to work, energy comes from four main wellsprings in human beings: the body, emotions, mind, and spirit. The core problem with working longer hours is that time is a finite resource. With remarkable consistency, these executives tell us they’re pushing themselves harder than ever to keep up and increasingly feel they are at a breaking point. We at the Energy Project have worked with thousands of leaders and managers in the course of doing consulting and coaching at large organizations during the past five years. That leads to declining levels of engagement, increasing levels of distraction, high turnover rates, and soaring medical costs among employees. Most of us respond to rising demands in the workplace by putting in longer hours, which inevitably take a toll on us physically, mentally, and emotionally. He slept poorly, made no time to exercise, and seldom ate healthy meals, instead grabbing a bite to eat on the run or while working at his desk. When we met him a year ago, he was working 12- to 14-hour days, felt perpetually exhausted, and found it difficult to fully engage with his family in the evenings, which left him feeling guilty and dissatisfied. Steve Wanner is a highly respected 37-year-old partner at Ernst & Young, married with four young children. When organizations invest in all dimensions of their employees’ lives, individuals respond by bringing all their energy wholeheartedly to work-and both companies and their people grow in value. These findings corroborated anecdotal evidence gathered about the effectiveness of this approach at other companies, including Ernst & Young, Sony, and Deutsche Bank. A group of Wachovia Bank employees who went through an energy management program outperformed a control group on important financial metrics like loans generated, and they reported substantially improved customer relationships, productivity, and personal satisfaction. The new workday rituals succeed only if leaders support their adoption, but when that happens, the results can be powerful. And participating in activities that give you a sense of meaning and purpose boosts the energy of the spirit. Avoiding the constant distractions that technology has introduced increases mental energy. Rejecting the role of a victim and instead viewing events through three hopeful lenses defuses energy-draining negative emotions. For instance, harnessing the body’s ultradian rhythms by taking intermittent breaks restores physical energy. In this article, Schwartz, founder of the Energy Project, describes how to establish rituals that will build energy in the four key dimensions. It has four wellsprings-the body, emotions, mind, and spirit-and in each, it can be systematically expanded and renewed. Time is a finite resource, but energy is different. Increasing that capacity is the best way to get more done faster and better. Meanwhile, people take for granted what fuels their capacity to work-their energy. As the demands of the workplace keep rising, many people respond by putting in ever longer hours, which inevitably leads to burnout that costs both the organization and the employee. Overall this has been an incredibly frustrating experience and I am so grateful for Reaper's flexibility, ease of use and customization. I then got another popup saying that 32 bit plugins are no longer supported and some of my plugins have been added to the blacklist. When I tried to start a new project, it said my ports were unmapped. Another thing I noticed was that the text was all blurry and wasn't in the correct resolution. Next, when Cubase had finally been installed and activated, a warning popped up saying it couldn't activate my Waves plugins. I'm not exaggerating when I say that it took 5-10 minutes for the activation process to finish, since it took a long time to update the activation license database. So I installed it and lo and behold, one of the first things was an incredibly long and frustrating activation process, even for a free license. So I saw that Cubase was offering a free 60 day license to encourage musicians to stay home which is great. We'd love to hear what you have produced with Reaper, but please post it in the weekly sticky. No piracyĭo not ask for or link to pirated content or pirate sites, and do not promote or suggest piracy. No unapproved commercial promotionĬommerical promotion by or on behalf of a commercial interest must be approved by moderators. If a difference of opinion becomes uncivil or unproductive, moderators may step in. We can have cooperative disagreements when we're trying to help each other. Memes and similar content are also considered off-topic. Posts should be related to Reaper, the digital audio workstation and MIDI sequencer software created by Cockos. We are read-only in protest of Reddit Inc disregarding its users and moderators. (This was in response to ads that had clearly specified a price, and some that had a link to pictures. Trade off is a heavier battery and bigger ESC.Send me the picture and the last reply to ads that already show a price, and don't mention a picture, this lazy bugger who didn't even fill in the subject with something about the ad is a pathetic giveaway.)ĭo you still have it ? I am interested. High rpm (high kv) is less efficient than a low Kv, higher voltage set up. Measuring efficiency as voltage changes for different Kv motors. Bigger diameter motors procude more torque (basically more leverage), but so to longer motors. Motor size and torque: Diameter vs length. Volt and amps can change, but V*I won’t change. The same motor at different Kvs doesn’t change the output torque. Kt, torque, volts, amps video: Kt = 1/kv (torque constant) not the same as actual torque: Torque = kt*I. Lower KV = more windings = supports higher voltage = smaller wire = more resistance (and waste heat/power loss) = same power at less amps. KV and efficiency video: Motor resistance (ohms) waste heat = P=I*I*R. It’s worth checking out to get an idea of acceptable combinations. Still, the e-props folks match multiple props to each specific IC and electric motor. Unfortunately, the the 36”-44” range of HG eharness props are 10-20% smaller than powered paramotor props–meaning we can’t leverage PPG forums and vendor data as information sources. Sensorless motors are lighter, less complicated, and less prone to failure. ESC manufactures improve efficiency and performance by using sensorless synchronization at high speed to alter the timing for the sequence of power pulses sent to the motors windings. While sensorless brushless motors perform poorly at low speed, their performance at flying speeds is excellent. Low cost custom KV: MAD motor, Alien Power Systems/FreerchhobbyĬharles Allen reports that APS provided a custom motor with a hollow shaft for ~$25 extra so he could use a rod to articulate a variable pitch prop. KV reduction Ĭustom rewinds change the KV rating and RPM. Probably useful to know if one of the larger diameter 35kv or 27kv motors marketed for use on paramotors is being considered. There is also a suggestion that the lower the no load kv value of the motor the greater the 100% duty cycle reduction factor. You need to determine this yourself or accept the numbers a manufacturer provides for a specific propeller.Įdit or copy the database Motor spec reality check You should have already calculated what you need for example: 25Kg of thrust at 50-70% throttle for 5 climb minutes with 12s batteries to climb to 1000’. You’ll have to use a combination of math, data table reading, and dice rolling to formulate an idea about what’s likely true. Dynamic thrust (when moving through the air) is far less. Thrust values derive from static thrust on a test stand with a specific prop. Some vendors user 1 minute, others 2 to 5 minutes or more. For example, “continuous current” doesn’t mean much unless you know the time frame. It’s worth checking Alibaba, Amazon, eBayĭon’t blindly trust published data or what’s in the table below. For example, APS sells Freerchobby motors. Some motors are marketed by others at higher prices. DC power trains are limited to max continuous power limitations (heat). Note the following:įor reference: Mosquito 10.4 kw, Wasp 10.4kw, Mossi 11.2kw: but an IC engine has a 100% duty cycle and can continuously run at full throttle. Unfortunately, comparing motors based on published specs is as difficult as walking up an Escher staircase. Other items you might care about are beyond the scope of this document, including thickness of the magnet laminations, magnet type, number of poles, wire pressure test, max temps for wire and magnets, etc. Internal resistance: Lower is better since resistance = heat. Lower KV motors have more permanent magnets to increase torque. The number before the letter N is the quantity of electromagnets in the stator, and the number before P is the quantity of permanent magnets in the motor. N and P numbers: something like “36N30P”. Design a system that does not exceed the max current for the short time specified by the manufacturer (often 60 seconds). Max current: How many burst amps you can draw at full throttle. It’s a good idea to design a system that’s operates around 50-70% of max current during climb to reduce heat and allow for power headroom. You may need “continuous” for your climb duration. Data sources are sketchy,Ĭontinuous current: How many amps the motor can handle for the number of minutes specified by the manufacturer until it overheats. You don’t have to do it naked, but you do have to do it. TBD: Intro Motor specification wrestling Portable toilet systems (WAG bags/Rest Stop 2 are great for backpacking, car camping, rafting, etc. Do not put cans, bottles, aluminum foil, or other non-burnable trash into a fire ring.Make sure your campfire is completely out (Drown it!) before you leave.So there is a VERY small chance that you will be able to find dead and down wood in this area!). DO NOT CUT LIMBS OFF TREES OR ENTIRE TREES DOWN FOR FIREWOOD! Please pack in firewood (there are around 70,000 visitors to this area each summer. Use only dead and down wood for campfires.Transfer the cold ashes into a plastic bag or other container and pack them out. Use existing rings or bring firepans (a metal tray with sides used to contain a campfire and prevent the fire from blackening the soil- oil pans work great!). There are two group campsites (reservations can be made by calling 97) which include one vehicle.Ĭamping near the warm springs is allowed but if resource damage to the area continues, this area could become a no-camping area. All other vehicles must pay per vehicle, per day. Individual campsites (first come, first served) and include one vehicle. There is also a developed fee campground at the Radium Recreation Site (1/2 mile from Mugrage) which offers a tent pad, picnic tables, fire rings, and toilets. You’ll need to possess a hunting or fishing license to camp here. There are primitive dispersed campsites at Mugrage Campground which is now a Colorado state wildlife area. Radium hot springs combine hot mineral water (affiliate) from underground, with cold Colorado River water, creating these unique warm springs. It is not uncommon to spot wildlife, rafters, and fishermen. There is one main pool, about twenty feet in diameter. This primitive warm spring is located on the Colorado River. It’s also allowed in other areas that BLM manages. Camping is permitted at the parking area at the base of the trail. The quickest way is actually by raft or kayak.Ĭliff jumping and tubing are popular on the Colorado River, as are commercial rafting trips, which make use of the springs during the summer season. There is a short hike to reach the springs from Warm Springs Road. Walk-in access available from Warm Springs Road located along Trough Road (CRI) between Pumphouse Recreation Site and Radium Road.” Radium Hot Springs sign at trailhead in 2022. No parking is permitted along radium road. Primitive Hot Springs Pool by Colorado River Rainbow Hot Springs near Kremmling. It is extremely dangerous and has resulted in injuries. Exercise caution if you choose to jump off the rocks. There are rocks across the river from the springs that visitors occasionally jump off. When the river is high, usually in the spring, the pool can be washed out. The spring mixes with river water bringing the temperature down to about 80⁰ F. The closest city to Radium to the north is Kremmling and to the south is Eagle and Edwards. Radium Hot Springs is a primitive warm springs pool located on the banks of the Colorado River. View this gist and download the instructions from GitHub.Send feedback and/or questions to me at.Your VPN should now initialize without prompting you for your username/password. Start OpenVPN again: sudo openvpn 'server-config-filename-goes-here.ovpn'.If you have OpenVPN running in another terminal tab halt that process with Ctrl+C.The modified line should now read as follows: auth-user-pass auth.txt.On the line which says " auth-user-pass" append the username/password filename " auth.txt".sudo vim 'server-config-filename-goes-here.ovpn' - Edit your chosen region's config file.Type your username on line 1, password on line 2 nothing more.Store Your Username and Password and Have OpenVPN Load Them Automatically sudo openvpn 'server-config-filename-goes-here.ovpn'.ls -l - List all server config files choose a region.sudo unzip openvpn.zip (install 'unzip' first if needed).You will be prompted to enter your PIA username and password after step 6 have those ready.This worked on my machine running openSUSE LEAP 42.1.This set-up has been known to work with several unsupported versions of Linux, as well as the supported Ubuntu.Use the following instructions to install OpenVPN via the command line in Linux.Menu OpenVPN & Private Internet Access Setup Tutorial 03 June 2016 on openvpn, private internet access, setup, tutorial, gist, VPN, PIA, store username and password, CLI Linux Command Line Setup of OpenVPN and Private Internet Access morpphling's Vampires, $2.25, 1st place on Event 285.mihahitlor's Warriors, $1.95, 1st place on Event 233.SirFalcon2008's Goblins, $1.54, 1st place on Event 289.The Top 10 Cheapest Decks that Went Undefeated Here's the prices of all the featured decks, courtesy of the Deck Pricer from mtgGoldfish ( MTGO Traders prices as of August 14, 2020): I mean, it's the tribe that directly references the creators of the game! And Bandit Keith is on his way to become the most successful Tribal Apocalypse player in history (two wins away from mihahitlor). It somewhat feels right that the 500th event was won by Wizards. Event link (with all players, pairings, standings, decks, and results): here it is.Tribes: Eldrazi, Elf (x2), Goblin (x2), Human, Spirit, Vampire (x3), Wizard (x2).Innovation Prize (tier 2): "Persistent Spirits" by lovetapsmtg and "500 Cards for 500 Events" by Nagarjuna.Innovation Prize (tier 1): "Cryptic Eldrazi" by Dawwy.1-loss: -DiamondDust- with Goblin, romellos with Vampire, ThyShuffler with Wizard.Subformat: 500th Event Special! (Legacy, only Top 10 tribes allowed).In the meantime, a big thank you to all of you. I'll remember some of them later in the year, when we actually cross the ten-year threshold in December. You guys are too many to mention, almost a hundred different winners, many hundreds of different participants. Ten years have passed, familiar names have come and gone, some of them have left a mark in the tournament's Hall of Fame. All of you who have come playing this semi-forgotten, MTGO-only format week after week. We have to be eternally grateful to all of them.īut I am grateful to all the players too. Without these people, there wouldn't be a Tribal Apocalypse, not for one week, and certainly not for 500. Then on January 8, 2011, Volker Kirstein aka BlippyTheSlug initiated the Blippian Era, from which we number the events. Later, host duties passed to Simon Smith aka Flippers_Giraffe. Its creator and host was Matthew Banister aka ShardFenix. That first event was held on September 19, 2009. The very first Tribal Apocalypse article on PureMTGO, by ShardFenix, about the very first event with that name, predating the Blippian Era.The Tribal Apocalypse article by KaraZorEl that inspired this series, about Tribal Apocalypse 39 (Endangered Species Week!).The first ever Diaries of the Apocalypse: Tribal Apocalypse 40.Here are the Diaries of the Apocalypse articles for the previous milestone events. Let's take a trip down memory lane, proceedin backwards. 1 win: Artificer, Atog, Avatar, Centaur, Djinn, Dog, Druid, Dwarf, Efreet, Giant, God, Hound, Illusion, Imp, Insect, Jackal, Jellyfish, Kavu, Lhurgoyf, Mutant, Nightmare, Pirate, Rebel, Rogue, Serpent, Shapeshifter, Siren, Skeleton, Slug, Spellshaper, Sphinx, Troll, and Wurm.2 wins: Cleric, Gargoyle, Hydra, Kobold, Naga, Nissa, Satyr, Treefolk, Vedalken, and Werewolf.3 wins: Demon, Dragon, Fish, Golem, Horror, Myr, Praetor, Snake, and Wolf.5 wins: Ally, Archon, Devil, Dinosaur, and Soltari.6 wins: Beast, Bird, Faerie, Kithkin, and Rat.8 wins: Scarecrow, Sliver, Soldier, and Warrior.9 wins: Angel, Berserker, Kor, Plant, and Wall. Demon event on week 75 awarded the win to both tribes, can't remember why). We can break down the remaining 490 weeks this way (the Angel vs. Also, 9 of the weeks were devoted to the Invitational, which doesn't crown a single tribe as the winner, while in Event 362, weirdly enough, nobody was the winner, due to extremely low attendance. With a caveat: during the first few seasons we didn't use a top cut, so the winners were calculated only based on the tie-breakers. To celebrate, here's the list of all the tribes that won an event in these 500 weeks. We did it! We reached the 500 events milestone! Woot! Check the full archive for the "Diaries of the Apocalypse" series This End-User License Agreement (this “Agreement”) is a legal document that contains the terms and conditions under which limited use of certain Software (as defined below) that operates with the Product is licensed to you. Now, finally, you will get the WeChat main chatting screen from you can add other contacts those are using the same chatting to communicate.Belkin International, Inc., including all affiliates and subsidiaries (“Belkin”, “us” or “we”) thanks you for choosing one of our Belkin, Linksys or Wemo products (the “Product”). Note: The security picture may be different in your case. But it is very simple you just need to drag the slider to match the missing piece of the photograph appears on the screen. The security check of the Wechat might look, new user, a bit complicated. To pass the security check Tap on the Start button. Although the app automatically grabs the code but in case it not then enter manually.Ĭheck the box given at the front of I have read and agree to the above terms text and tap on the NEXT button.Īfter all above steps, Wechat app will start security check verification to confirm the account is created by human only. In a few seconds, you will receive the Wechat verification code. To Secure the Wechat account assigns some password and then finally tap on the Sign-up button.Īt this step the We chat app will ask for the permission to send the message (SMS) to get the verification code from the We Chat server. And next to Region column enter the phone number with which you want to register the Wechat account. For example, I want to sign up WeChat in India because currently, my mobile number is from Indian Telecom. First, we enter the username, then select the Region of where SIM card or mobile number you are using. That’s why tap on the Signup button.Īt the step of Wechat account creation, we need to enter our information just like WhatsApp. If you already have the Wechat account then simply go for the Login button but I am sure you are here because you don’t have the one. On the first screen of the Wechat you will find two options one is Sign up and other is Log in. Step 2: Sign up WechatĪfter installing the app, open it. You can also directly open your respective play store on your smartphone and search for Wechat to download and install it. If you are Android user then use this link: Google Play store and for iPhone/iPad users here is the App store link. To signup the Wechat first we need to download that. Step 9: WeChat main chatting screen Signup Wechat personal account on Android or iPhone Step 1: Download the Wechat app on Smartphone If you want to test the Wechat then here is the tutorial to know how to create an account and use it. The Wechat offers two online websites one is mp.weixin.qq.com which is in Chinese language and other for rest of the world to download the Wechat app for Android and iPhone (iOS) devices. Although the Chinese government banned foreign application, their apps can be access or available for the rest of the world. Though it is not that much popular in the rest of the world as it is in China.Īs we know in China most of the world social media channels and apps are banned, and because that their own homegrown company Tencent had developed the WeChat to provide the WhatsApp like features or attributes while following the country’s security protocols. However, one more chatting application called Wechat completely has dominated China also a popular one among the chatting applications in the world. The apps like WhatsApp, Hike, Telegram are few top international apps those used by a lot of people to chat and share the information in their daily routine. “ Digital chat is now at its zenith and almost dominated the verbal communication of human being“. In this tutorial, we let you know how to create a Wechat personal account and clear the security check on Android and iPhone/iPad smartphones. Note: If you like our work order template, you should also check out our free HVAC invoice template. How to Download and Use Our HVAC Work Order Template Want to see how ServiceTitan can increase efficiency and grow your profits? Schedule a call for a free product tour. Our HVAC software helps businesses like yours integrate their work order processes with dispatching, invoicing, and more. And then we’ll explain how ServiceTitan streamlines the entire work order creation process, and connects with other parts of your business. īelow, we’ll walk you through how to download and use our free template. » Want to grow your HVAC business? Click here to get a demo. To solve these challenges, a growing number of business owners are using HVAC software like ServiceTitan to create accurate, complete, and easy-to understand HVAC service orders that automatically integrate with invoicing and the rest of your operation. Turning work orders into invoices when it’s time to get paid Inefficiencies in call booking and dispatching Miscommunication between office staff and techs in the field But although it’s a great starting point, it doesn’t solve the process-related issues involved with creating, tracking, and managing work orders.įor example, regardless of whether it’s in print or digital form (Excel, Microsoft Word, etc.), a template alone does not solve challenges with: Having a template allows you to be consistent in the information you collect about jobs. It’s been created by industry experts on our team to include the essential fields your techs and CSRs need. To help your staff collect the necessary information for performing HVAC jobs, we’ve designed an HVAC work order template that’s free for you to download. |
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